王偉博士
- 基本信息
- 教育經(jīng)歷
- 工作經(jīng)歷
- 研究概述
- 發(fā)表文章
王偉 博士北京生命科學(xué)研究所研究員Wei Wang, Ph.D.Assistant Investigator, NIBS, Beijing，ChinaEmail: wangwei@nibs.ac.cnHome page: https://killifishlab.com/
Ph.D. in Biology, The University of Alabama, USA
M.S. in Genetics, Northwest A&F University, Yangling, China
B.S. in Biology and B.E. minor in Computer Science and Technology, Northwest A&F University, Yangling, China.
2021-Present 北京生命科學(xué)研究所研究員
Assistant Investigator, National Institute of Biological Sciences, Beijing, China
2014-2021年 美國(guó)斯托瓦斯醫(yī)學(xué)研究所、霍華德·休斯醫(yī)學(xué)研究所博士后
Postdoctoral Research Fellow, Stowers Institute for Medical Research and Howard Hughes Medical Institute, Kansas City, USA
器官原位再生在幫助人類恢復(fù)因損傷、疾病、衰老等造成的器官功能減弱或喪失方面具有巨大潛力，是生物醫(yī)學(xué)領(lǐng)域亟待解決的重大科學(xué)問題。在自然界中，部分低等脊椎生物（例如魚類、蠑螈等）具有極強(qiáng)的器官再生能力，他們可以完美地修復(fù)受損的心臟、大腦、脊髓及肢體；相比之下，哺乳動(dòng)物（包括人類）卻喪失了這些能力。決定不同器官再生或不能再生的分子機(jī)制、以及如何讓哺乳動(dòng)物重新獲得器官再生能力仍是領(lǐng)域內(nèi)未解的難題。以哺乳動(dòng)物耳廓損傷為研究模型，我們率先發(fā)現(xiàn)了決定器官再生能力的“分子開關(guān)”的存在，關(guān)閉“開關(guān)”會(huì)導(dǎo)致再生物種喪失再生能力，而重啟“開關(guān)”可以激活不可再生器官的再生能力 。這一發(fā)現(xiàn)為解析哺乳動(dòng)物脊髓等其他核心器官再生失敗的分子機(jī)制提供了新思路。借助具有顯著優(yōu)勢(shì)的短壽非洲鳉魚（Nothobranchius furzeri）模型及具有強(qiáng)大遺傳學(xué)工具的小鼠模型，王偉實(shí)驗(yàn)室的主要研究興趣是鑒定賦予器官再生能力的核心分子機(jī)制，并以其為靶標(biāo)通過再生醫(yī)學(xué)手段探索人類受損器官的重建。
非洲鳉魚棲息在非洲東南部遭受季節(jié)性干旱的臨時(shí)水塘中。成年的非洲鳉魚僅在雨季池塘蓄水時(shí)才會(huì)出現(xiàn)。在干旱無水的季節(jié)，非洲鳉魚以被埋在泥中且處于滯育（diapause）或休眠狀態(tài)的胚胎形式存活，等待下一個(gè)雨季孵化并再次繁殖。強(qiáng)大的自然選擇壓力使得該物種進(jìn)化出了獨(dú)有的特征，這些特征可以大大加速器官發(fā)育、再生及衰老相關(guān)的研究：1）生長(zhǎng)及性成熟速度快（野外14天即可性成熟，實(shí)驗(yàn)室條件下30-45天可性成熟），極大地節(jié)省了遺傳操作和實(shí)驗(yàn)時(shí)間； 2）在實(shí)驗(yàn)室內(nèi)胚胎可以進(jìn)入滯育或休眠長(zhǎng)達(dá)5-6年，使得遺傳品系的長(zhǎng)期保存方便且費(fèi)用低，無需像其他動(dòng)物模型需要長(zhǎng)期飼養(yǎng)成年動(dòng)物； 3）在實(shí)驗(yàn)室條件下衰老速度極快（平均壽命為3-5個(gè)月），是目前實(shí)驗(yàn)室可飼養(yǎng)的壽命最短的脊椎動(dòng)物模型； 4）擁有高效的遺傳操作技術(shù)：CRISPR/Cas9基因敲除及敲入、Tol2介導(dǎo)的轉(zhuǎn)基因等?？傊侵搠汈~為成年動(dòng)物器官發(fā)育、再生和衰老研究帶來了傳統(tǒng)模型所缺乏的顯著優(yōu)勢(shì)和全新機(jī)遇。
Regeneration has long attracted biomedical interest because of the potential of replacing damaged organs with new ones. However, why some lower vertebrates (e.g., fish and salamanders) regenerate extensively while others such as mammals regenerate poorly is not well understood. In addition to the popular mouse model, the Wang lab is also powered with a new genetic model, the African killifish Nothobranchius furzeri. We are deploying comparative studies and genetic screens to decode the failure of regeneration in non-regenerative animals. The Wang Lab is interested in identifying molecular mechanisms that can be targeted to help humans rebuild damaged organs. Our current research will focus on, but not be limited to, the following areas:
(1) The molecular basis of spinal cord regeneration.
(2) Evolution of regenerative capacities in vertebrates.
(3) Regeneration and Rejuvenation.
Wang Lab website: https://killifishlab.com/
Representative Research Articles:
1. Lin, W., Jia, X., Shi, X., He, Q., Zhang, P., Zhang, X., Zhang, L., Wu, M., Ren, T., Liu, Y., Deng, H., Li, Y., Liu, S., Huang, S., Kang, J., Luo, J.#, Deng, Z.#, Wang, W.#, 2025. Reactivation of mammalian regeneration by turning on an evolutionarily disabled genetic switch. Science 388, eadp0176. 10.1126/science.adp0176.
2. Zhang, JQ., Zhou YQ., Yue, W., Zhu ZS., Wu XL., Yu, S., Shen QY., Pan Q., Xu, WJ., Zhang, R., Wu, XJ., Li, XM., Li, YY, Li, YX., Wang, Y., Peng, S., Zhang, SQ., Lei, AM., Ding, XB., Yang, F., Chen, XQ., Li, N.#, Liao, MZ.#, Wang, W. #, Hua, JL#, 2022. Super-enhancers conserved within placental mammals maintain stem cell pluripotency, Proc Natl Acad Sci U S A, 119 (40) e2204716119.
3. Wang, W., Hu, C.-K., Zeng, A., Alegre, D., Hu, D., Gotting, K., Ortega Granillo, A., Wang, Y., Robb, S., Schnittker, R., Zhang, S., Alegre, D., Li, H., Ross, E., Zhang, N., Brunet, A., Sánchez Alvarado, A., 2020. Changes in regeneration-responsive enhancers shape regenerative capacities in vertebrates. Science 369, (10.1126/science.aaz3090).
Research Highlights in Nature: Why some animals have the power of regeneration. https://www.nature.com/articles/d41586-020-02529-5
Research Highlights in Nature Reviews Genetics: Enhancing regeneration.
https://www.nature.com/articles/s41576-020-00290-z
4. Hu, C.K., Wang, W., Brind'Amour, J., Singh, P.P., Reeves, G.A., Lorincz, M.C., Alvarado, A.S., Brunet, A., 2020. Vertebrate diapause preserves organisms long term through Polycomb complex members. Science 367, 870-874.
5. Cao, C., Lemaire, L.A., Wang, W., Yoon, P.H., Choi, Y.A., Parsons, L.R., Matese, J.C., Wang, W., Levine, M., Chen, K., 2019. Comprehensive single-cell transcriptome lineages of a proto-vertebrate. Nature 571, 349-354.
6. Zeng, A., Li, H., Guo, L., Gao, X., McKinney, S., Wang, Y., Yu, Z., Park, J., Semerad, C., Ross, E., Cheng, L.C., Davies, E., Lei, K., Wang, W., Perera, A., Hall, K., Peak, A., Box, A., Sanchez Alvarado, A., 2018. Prospectively Isolated Tetraspanin(+) Neoblasts Are Adult Pluripotent Stem Cells Underlying Planaria Regeneration. Cell 173, 1593-1608 e1520.
7. Wang, W., Tindell, N., Yan, S., Yoder, J.H., 2013. Homeotic functions of the Teashirt transcription factor during adult Drosophila development. Biology open 2, 18-29.
8. Wang, W., Yoder, J.H., 2012. Hox-mediated regulation of doublesex sculpts sex-specific abdomen morphology in Drosophila. Dev Dyn 241, 1076-1090.
9. Wang, W., Kidd, B.J., Carroll, S.B., Yoder, J.H., 2011. Sexually dimorphic regulation of the Wingless morphogen controls sex-specific segment number in Drosophila. Proc Natl Acad Sci U S A 108, 11139-11144.
10. Wang, W., Yoder, J.H., 2011. Drosophila pupal abdomen immunohistochemistry. J. Vis. Exp. (56), e3139, doi:10.3791/3139
Book chapters and Protocols:
1. Wei Wang #, Nicolas Rohner #, and Yongfu Wang #. Book Series: Emerging Model Organisms, Neuromethods, volume 194, volume 194, 2023 (https://doi.org/10.1007/978-1-0716-2875-1). (# Corresponding Editors).
2. Yufei Lou, Weifeng Lin , Wei Wang, 2023. Efficient Transgenesis in African Turquoise Killifish Using a Gibson Assembly–Based Tol2 Transposon System. Cold Spring Harb Protoc.
3. Ortega Granillo, A. , Schnittker, R. , Wang, W #., and Alvarado, A. S #.(2023). Quantifying cell proliferation through immunofluorescence on whole-mount and cryosectioned regenerating caudal fins in African killifish. Bio Protoc. Dec 20;13(24):e4908.
